Singlepath® E. coli O157 – Rapid Pathogen Screening
Brand Chemical Supplies offers the Singlepath® E. coli O157 lateral flow test, a gold-standard rapid screening tool for the qualitative detection of Escherichia coli O157 in food samples. This “gold-standard” immunological assay is designed to provide definitive results with the speed of a pregnancy test, significantly reducing the hold-time for perishable goods and batch releases in high-volume food production environments.
Technical Specifications
| Feature | Specification |
| Method | Lateral Flow Immunochromatographic Assay |
| Target Pathogen | Escherichia coli O157 (including H7) |
| Detection Limit | 1 colony-forming unit (CFU) per 25g sample (after enrichment) |
| Test Time | 20 Minutes (post-enrichment) |
| Compliance | AOAC Performance Tested / AFNOR Certified |
| Shelf Life | Store at 2 \sim 8 °C |
Key Features & Benefits
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Rapid Turnaround: While traditional culture methods can take $3 \sim 5$ days to confirm O157 presence, Singlepath® provides a visual result in just 20 minutes following the initial enrichment period.
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High Specificity: Utilizes highly specific antibodies to minimize cross-reactivity with non-O157 E. coli or other enteric bacteria, reducing the frequency of “false positives” that can disrupt production.
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Simplified Workflow: The test requires only a few steps. After sample enrichment, simply apply the sample to the test port. No specialized expensive equipment (like PCR machines) is required to read the results.
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Built-in Control: Every test strip includes an integrated control line (C), ensuring the test has functioned correctly and the results are valid.
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Easy Interpretation: Clear visual lines (one for negative, two for positive) make it easy for laboratory technicians to make quick, definitive “go/no-go” decisions on batches.
Primary Applications
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Meat & Poultry Processing: Screening raw ground beef, carcass swabs, and trim for O157:H7 contamination.
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Fresh Produce: Testing leafy greens, sprouts, and unpasteurized juices.
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Dairy Industry: Monitoring raw milk and cheese production environments.
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Environmental Swabbing: Verifying the cleanliness of food contact surfaces and equipment after sanitation cycles.
Testing Protocol Summary
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Enrichment: Incubate the $25g$ food sample in a suitable enrichment broth (e.g., Modified TSB with Novobiocin) for $18 \sim 24$ hours.
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Sample Application: Transfer a small aliquot of the enriched broth to the Singlepath® test device.
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Read Results: Observe the test window after 20 minutes. Two lines indicate a positive result for E. coli O157, requiring immediate regulatory action or further confirmation.
Contact our microbiology technical team at Brand Chemical Supplies today to secure your Singlepath® E. coli O157 kits and enhance your facility’s pathogen intervention program
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